Document Type : Original Research Article
Authors
1
PhD Student in Clinical Biochemistry, Faculty of Medicine, University of Baghdad, Baghdad, Iraq
2
Assistant Professor, Clinical Biochemistry, Department of Biochemistry, College of Medicine, University of Baghdad, Baghdad, Iraq
10.22038/jmrh.2024.75923.2239
Abstract
Background & aim: Polycystic ovary syndrome (PCOS) is the most common prevalent endocrine disorder in women of childbearing age. Insulin resistance present in 50%-80% of PCOS cases and is exacerbated by the accumulation of adipose tissue associated with hyperandrogenism. Metforminis associated with a positive improvement in menstrual cycles and body mass index. The objective of this study was to assess the role of androgen profile, insulin resistance, and adiponectin to evaluate the benefits of combined vitamin D and metformin in the treatment of PCOS.
Methods: This cross sectional study was conducted in Baghdad, Iraq, from September 2022 to June 2023, involved 50 infertile females diagnosed with PCOS (age: 18-40 years) divided into two sub-groups. Group I (n=25) received metformin (850 mg/twice daily) and followed for 8 weeks. Group II (25 women) received metformin (850 mg/twice daily) plus vitamin D3 (50.000 IU/wk) for 8 weeks. Serum investigations included evaluation of dihydrotestosterone, free testosterone, sex-hormone binding globulin, dehydroepiandrosterone-sulfate, 25-hydroxyvitamin D, HOMA-IR, and adiponectin via ELISA. Data analysis was performed using SPSS software (version 23.0).
Results: The mean values of free testosterone (3.22 ± 0.42 pg/ml, 4.24 ± 0.28 pg/ml, P<0.001), dihydrotestosterone (450 ± 33.25 pg/ml, 496 ± 15.88 pg/ml, P<0.001), and waist circumference (93.22 ± 10.00 cm, 99.76 ± 11.68 cm, P<0.021) were significantly lower after treatment with combined metformin plus vitamin D, compared to those after treatment with metformin alone. Moreover, the mean value of adiponectin was significantly increased after the combined treatment, compared to that after metformin alone (4.25 ± 0.39 ng/ml, 2.49 ± 0.29 ng/ml, P<0.001).
Conclusion: Considering the PCOS women treatment, serum measurements of free testosterone, dihydrotestosterone, and adiponectin are good indicators for the efficacy of combined metformin plus vitamin D supplement. Combined treatment was superior to that of metformin alone in the treatment of PCOS.
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